An ELISA for the quantification of chicken immunoglobulin (IgY) in various liquid media
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Abstract
The development of an assay for the quantification of chicken Immunoglobulin (IgY) and data evaluation is being demonstrated. An enzyme immunoassay was established to perform the quality control of IgY producing cells in vitro. Each step of the test procedure and possible alternatives were described. The assay was characterised by special test parameters (sensitivity, detectability, accuracy, precision, specifity). Moreover, this simple and fast enzyme immunoassay is a special supplement to the alternative antibody production from egg yolk.
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