Highly sensitive detection of cancer antigen 15-3 using novel avian IgY antibodies

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Renata Grzywa
Agnieszka Łupicka-Słowik
Maciej Walczak
Magdalena Idzi
Kamila Bobrek
Stephane Boivin
Andrzej Gaweł
Tadeusz Stefaniak
Józef Oleksyszyn
Marcin Sieńczyk


Early detection of cancer development is crucial for successful therapy and for monitoring patient outcome. Various immunodiagnostic methods are able to detect pathological changes in the human body ahead of symptomatic manifestation of the disease. Most immunological examinations are based on the detection of specific tumor markers in body fluids. Of the various cancer-specific proteins used for breast cancer diagnostics, one of the most commonly applied is the cancer antigen 15-3 (CA 15-3). An elevation in its serum level (>25-40 U/ml) usually correlates with tumor malignancy. The CA 15-3 antigen is also used for monitoring patients after surgical treatment and for measuring therapeutic efficacy. Herein, we present the generation of polyclonal IgY antibodies isolated from egg yolks of immunized hens and their application for CA 15-3 detection. The developed sandwich ELISA assay showed a detection limit of 0.028 U/ml, thus demonstrating its potential for clinical applications.

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Grzywa, R., Łupicka-Słowik, A., Walczak, M., Idzi, M., Bobrek, K., Boivin, S., Gaweł, A., Stefaniak, T., Oleksyszyn, J. and Sieńczyk, M. (2014) “Highly sensitive detection of cancer antigen 15-3 using novel avian IgY antibodies”, ALTEX - Alternatives to animal experimentation, 31(1), pp. 43–52. doi: 10.14573/altex.1309181.