Organ-on-a-chip (OoC) technologies are promising tools to reduce the reliance on animal models in basic and applied research. The NC3Rs has created a new resource bringing together their OoC activities as well as other global programmes and initiatives. As well as showcasing the NC3Rs' portfolio of reports and projects in this area, the resource provides information and support for the OoC community, including technology developers, end-users, regulators and in vivo researchers who are looking to adopt alternative approaches.